Analysis Note
Control3T3/A31 cell lysate
Application
Use Anti-Cyclin D Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IP to detect Cyclin D also known as B-cell CLL/lymphoma 1, BCL-1 oncogene.
Research CategoryEpigenetics & Nuclear Function
Research Sub CategoryCell Cycle, DNA Replication & Repair
Western Blot (SNAP ID) Analysis: 5 µg/ml from a previous lot detected Cyclin D on 10 µg of 3T3/A31 cell lysate.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cyclin D in NIH/3T3, A431, C2C12, and HeLa cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Progression through G1 phase and transition from G1 to S phase of the cell division cycle is controlled by activation of a distinct series of serine/threonine kinase complexes which comprise of a cyclin regulatory subunit and a cyclin dependent kinase (cdk). D type cyclins are induced earlier than cyclin E in G1 and can form complexes with cdk2,-4,-5, and -6. Cyclin D1 is a nuclear protein during G1 phase and disappears from the nucleus in S-phase. Among the key cell cycle regulators, cyclin D1 has been implicated most strongly as a proto-oncogene in several human tumor types, including breast carcinomas.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to human Cyclin D at the C-terminus.
Linkage
Replaces: 04-221
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality
Evaluated by Western Blot in 3T3/A31 cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Cyclin D on 10 µg of 3T3/A31 cell lysate.
Specificity
This antibody recognizes Cyclin D at the C-terminus.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
~ 34 kDa
This product has met the following criteria to qualify for the following awards: